|
KMID : 0352519940310010063
|
|
Korea Univercity Medical Journal
1994 Volume.31 No. 1 p.63 ~ p.72
|
|
|
An Immunohistochemical Study of c-myc, c-h-ras, and p53 related Proteins in Normal,
|
|
|
|
|
Abstract
|
|
|
Activation of oncogenes, mutated genes, and inactivation of suppressor genes were
the major genetic abnormalities in carcinogenesis. Oncogene-related proteins can be detected by an immunohistochemical staining or other techniques in molecular biology. Accumulation of abnormal protein encoded from mutated p53
immunoreactivity is
usually not seen in normal cells because the protein has a very short half-life. Using monoclonal and polyclonal antibodies against form c-myc, H-ras, and p53 related proteins, the author has performed an experiment on the normal,
dysdlastic, and malignant gastric mucosa.
@ES The results obtained were as follows;
@EN 1. c-myc showed no expression on all the gastric mucosa used.
2. H-ras showed expression on the normal glandular epithelium, especially parietal cells,
peripheral to the dysplastic and neoplastic glands. And a few cases(29%) show cytoplasmic expression in signet-ring cells of gastric adenocarcinomas.
3. p53 showed nuclear expression in 7% of dysplastic mucosa, and 50% of gasric adenocarcinomas. However, there is no relationship with the expression and between the histologic differentiation and depth of tumor invasion.
With the above results, p53 alterations occur relatively late in carcinogenic process and there is different molecular biology between the gastric and the colorectal adenocarcinomas in which Ki-ras and p53 were frequenly expressed.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|